bsm-54389R [Primary Antibody]
GNL3 (6H5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GNL3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 26354

Swiss Prot: Q9BVP2

Source: Recombinant protein corresponding to the C-terminus of human Nucleostemin.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation. May interact with p53 and may be involved in tumorigenesis. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 62


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

IF(ICC) staining with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of A549 cells with GNL3 (6H5) Monoclonal Antibody (bsm-54389R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).