bsm-54390R [Primary Antibody]
Annexin IV (1G2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ANXA4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: /307

Swiss Prot: P09525

Source: Recombinant protein within human Annexin IV aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Background:

The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Ca++-dependent manner and a unique amino terminal region which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino terminus with possible Ca++ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl- flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 36


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HepG2 cells with Annexin IV (1G2) Monoclonal Antibody (bsm-54389R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Annexin IV (1G2) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Annexin IV (1G2) Monoclonal Antibody (bsm-54389R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with Annexin IV (1G2) Monoclonal Antibody (bsm-54389R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Annexin IV (1G2) Monoclonal Antibody (bsm-54389R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Annexin IV (1G2) Monoclonal Antibody (bsm-54389R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: Human Liver; Probed with Annexin IV (1G2) Monoclonal Antibody (bsm-54389R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.