bsm-54393R [Primary Antibody]
PICK1 Recombinant Antibody
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Host: Rabbit

Target Protein: PICK1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9463

Swiss Prot: Q9NRD5

Source: Recombinant protein within N-terminal human PICK1.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
FCM(1:20-100)
IP(1-2ug)

Predicted Molecular Weight: 47


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SK-Br-3 Cells; Probed with PICK1 (4G1) Monoclonal Antibody (bsm-54393R) at 1:5000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of 293T cells with PICK1 (4G1) Monoclonal Antibody (bsm-54393R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).