bsm-54402R
[Primary Antibody]
UPF1 (9A3) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: UPF1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5976
Swiss Prot: Q92900
Source: Recombinant protein within human hUPF1 aa 1-200.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 124
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: PC-3M Cells; Probed with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of PC-3M cells with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).