DATASHEET
Host:
Rabbit
Target Protein:
CDX1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
1044
Swiss Prot:
P47902
Source:
Recombinant protein of full lenth sequence of human Cdx1.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Plays a role in transcriptional regulation. Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells. Binds to the PRKD1 promoter in colorectal cancer (CRC) cells. Could play a role in the terminal differentiation of the intestine.
VALIDATION IMAGES
Lane 1: Human Small Intestine; Lane 2: CRC Cells; Probed with CDX1 (3D1) Monoclonal Antibody (bsm-54403R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CDX1 (3D1) Monoclonal Antibody (bsm-54403R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.