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Host: Rabbit
Target Protein: Carbonic Anhydrase I
Immunogen Range: 1-200/261
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 759
Swiss Prot: P00915
Source: Recombinant human Carbonic Anhydrase I
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Store at -20°C for 12 months.
Background:
Size: 100µL
Concentration: Lot Dependent
Applications:
WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:50-200)
IF(1:100-500)
ICC/IF(1:50-200)
Predicted Molecular Weight: 29
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with Carbonic Anhydrase I (2H4) Monoclonal Antibody (bsm-54409R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Small Intestine tissue incubated with Carbonic Anhydrase I (2H4) Monoclonal Antibody (bsm-54409R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Spleen tissue incubated with Carbonic Anhydrase I (2H4) Monoclonal Antibody (bsm-54409R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
25 ug total protein per lane of various lysates (see on figure) probed with Carbonic Anhydrase I monoclonal antibody, unconjugated (bsm-54409R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.