bsm-54410R [Primary Antibody]
IQGAP1 (2B5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IQGAP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8826

Swiss Prot: P46940

Source: Recombinant protein corresponding to N-terminal human IQGAP1.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 189


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of SiHa cells with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


IF(ICC) staining with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Lane 1: HUVEC Cells; Lane 2: A431 Cells; Lane 3: Mouse Placenta; Probed with IQGAP1 (2B5) Monoclonal Antibody (bsm-54410R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.