bsm-54413R-BF488

DATASHEET

Host: Rabbit

Target Protein: Bub3

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 9184

Swiss Prot: O43684

Source: Recombinant human Bub3

Purification: purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Conjugation: AbBy Fluor-488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC/IF(1:50-200)
IF(1:100-500)
Flow-Cyt(1:50-100)

Predicted Molecular Weight: 36 kDa

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.