bsm-54417R [Primary Antibody]
ADAMTS13 (1G9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADAMTS13

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 11093

Swiss Prot: Q76LX8

Source: Recombinant protein within human ADAMTS13 aa 150-350.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.

Storage Condition: Store at -20°C for 12 months.

Background: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SiHa Cells; Lane 2: PC-3M Cells; Lane 3: A549 Cells; Probed with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of HepG2 cells with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).