bsm-54423R [Primary Antibody]
Nectin 2 (3G7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NECTIN2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5819

Swiss Prot: Q92692

Source: Recombinant protein within human Nectin 2 aa 50-250.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Nectin 2 is a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Can form trans-heterodimers with PVRL3/nectin-3.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: K562 Cells; Lane 2: MCF-7 Cells; Lane 3: SK-OV-3 Cells; Probed with Nectin 2 (3G7) Monoclonal Antibody (bsm-54423R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Nectin 2 (3G7) Monoclonal Antibody (bsm-54423R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Nectin 2 (3G7) Monoclonal Antibody (bsm-54423R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with Nectin 2 (3G7) Monoclonal Antibody (bsm-54423R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Nectin 2 (3G7) Monoclonal Antibody (bsm-54423R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Nectin 2 (3G7) Monoclonal Antibody (bsm-54423R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of LOVO cells with Nectin 2 (3G7) Monoclonal Antibody (bsm-54423R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).