bsm-54424R [Primary Antibody]
CTBP1 (3A12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CTBP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1487

Swiss Prot: Q13363

Source: Recombinant protein within human CtBP1 aa 300-500.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

CTBP1 is a corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. CTBP1 also binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: PC-3M Cells; Probed with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with CTBP1 (3A12) Monoclonal Antibody (bsm-54424R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.