VALIDATION IMAGES
Lane 1: MCF-7 Cells; Probed with Retinoid X Receptor alpha (3B5) Monoclonal Antibody (bsm-54427R) at 1:5000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Retinoid X Receptor alpha (3B5) Monoclonal Antibody (bsm-54427R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Retinoid X Receptor alpha (3B5) Monoclonal Antibody (bsm-54427R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Liver tissue incubated with Retinoid X Receptor alpha (3B5) Monoclonal Antibody (bsm-54427R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Esophagus tissue incubated with Retinoid X Receptor alpha (3B5) Monoclonal Antibody (bsm-54427R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human A431 cell lysates probed with Retinoid X Receptor alpha Polyclonal Antibody, Unconjugated (bsm-54427R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.