bsm-54430R [Primary Antibody]
VAMP1 (4F7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VAMP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6843

Swiss Prot: P23763

Source: Full length recombinant protein of human VAMP1.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. VAMP1 is involved in the targeting and/or fusion of transport vesicles to their target membrane.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 13


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Cerebellum; Lane 2: Rat Brain; Probed with VAMP1 (4F7) Monoclonal Antibody (bsm-54430R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with VAMP1 (4F7) Monoclonal Antibody (bsm-54430R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with VAMP1 (4F7) Monoclonal Antibody (bsm-54430R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with VAMP1 (4F7) Monoclonal Antibody (bsm-54430R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with VAMP1 (4F7) Monoclonal Antibody (bsm-54430R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with VAMP1 (4F7) Monoclonal Antibody (bsm-54430R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.