bsm-54437R [Primary Antibody]
Lysyl-tRNA synthetase (1H8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KARS

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3735

Swiss Prot: Q15046

Source: Recombinant protein corresponding to C-terminal human Lysyl tRNA synthetase.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 68


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: SiHa Cells; Lane 2: MCF-7 Cells; Probed with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.