DATASHEET
Host:
Rabbit
Target Protein:
NXF1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
10482
Swiss Prot:
Q9UBU9
Source:
Recombinant protein within c-terminal human NXF1.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.
VALIDATION IMAGES
Lane 1: 293 Cells; Lane 2: SiHa Cells; Probed with NXF1 (1G8) Monoclonal Antibody (bsm-54439R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with NXF1 (1G8) Monoclonal Antibody (bsm-54439R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.