bsm-54440R [Primary Antibody]
PGAM1 (2G9) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PGAM1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5223

Swiss Prot: P18669

Source: Recombinant protein within N-terminal human PGAM1.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

PGAM1 catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A431 Cells; Lane 2: A549 Cells; Lane 3: Rat Brain; Lane 4: Mouse Brain; Probed with PGAM1 (2G9) Monoclonal Antibody (bsm-54440R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with PGAM1 (2G9) Monoclonal Antibody (bsm-54440R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with PGAM1 (2G9) Monoclonal Antibody (bsm-54440R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of Jurkat cells with PGAM1 (2G9) Monoclonal Antibody (bsm-54440R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).