DATASHEET
Host:
Rabbit
Target Protein:
CBS
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
875
Swiss Prot:
P35520
Source:
Recombinant protein within human CBS aa 400-550.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons
VALIDATION IMAGES
Lane 1: 293 Cells; Lane 2: 293 Cells; Lane 3: Human Liver; Probed with Cystathionine beta-synthase (1C2) Monoclonal Antibody (bsm-54448R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Cystathionine beta-synthase (1C2) Monoclonal Antibody (bsm-54448R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.