bsm-54452R [Primary Antibody]
PSENEN (1D8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PSENEN

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 55851

Swiss Prot: Q9NZ42

Source: Synthetic peptide corresponding to C-terminal human PEN2.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 12


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Spleen; Probed with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:2000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Rat Cerebellum tissue incubated with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Flow cytometric analysis of A549 cells with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).