VALIDATION IMAGES
Lane 1: Daudi Cells; Probed with DDB2 (5A3) Monoclonal Antibody (bsm-54454R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with DDB2 (5A3) Monoclonal Antibody (bsm-54454R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with DDB2 (5A3) Monoclonal Antibody (bsm-54454R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with DDB2 (5A3) Monoclonal Antibody (bsm-54454R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of A549 cells with DDB2 (5A3) Monoclonal Antibody (bsm-54454R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).