bsm-54457R [Primary Antibody]
SRP54 (1A4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SRP54

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6729

Swiss Prot: P61011

Source: Recombinant protein within human SRP54 aa 1-200.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

SRP54, a component of the signal recognition particle (SRP), recognizes the signal peptide of secretory proteins and interacts with the signal recognition particle receptor to target the ribosome and the associated nascent chain to the endoplasmic reticulum. By functional analysis showed that SRP54 binds to SRP RNA via the M-domain, but only in the presence of RNA-bound SRP19, and that it associates with the signal peptide of nascent polypeptide chains. RNA interaction requires the presence of a loop in the C-terminal M-domain. Signal peptide recognition most likely involves methionine-rich loops. During signal peptide recognition, SRP54 is positioned at the exit site close to ribosomal proteins L23a and L35. When SRP54 contacts the signal recognition particle receptor, SRP54 is rearranged such that it is no longer close to L23a. This repositioning may allow the translocon to dock with the ribosome, leading to insertion of the signal peptide into the translocation channel.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HepG2 Cells; Lane 2: MCF-7 Cells; Probed with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Flow cytometric analysis of HepG2 cells with SRP54 (1A4) Monoclonal Antibody (bsm-54457R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).