VALIDATION IMAGES
Lane 1: U937 Cells; Probed with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Flow cytometric analysis of HepG2 cells with FYB1 (1F10) Monoclonal Antibody (bsm-54461R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).