bsm-54462R [Primary Antibody]
MARK3 (2F7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MARK3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4140

Swiss Prot: P27448

Source: Recombinant protein within human Mark3 aa 600-750.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 84


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat Brain; Lane 2: A431 Cells; Lane 3: 293 Cells; Probed with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X100/TBS for 10 mins at room temperature.


IF(ICC) staining with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X100/TBS for 10 mins at room temperature.


IF(ICC) staining with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X100/TBS for 10 mins at room temperature.


Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Paraformaldehyde-fixed and paraffin-embedded Mouse Small Intestine tissue incubated with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.


Flow cytometric analysis of MCF-7 cells with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).