bsm-54463R [Primary Antibody]
PPP1CB (6G6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PPP1CB

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5500

Swiss Prot: P62140

Source: Recombinant protein corresponding to human PPP1CB aa 100-327.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse skeletal muscle; Lane 2: Rat brain; Lane 3: A431 Cells; Lane 4: SH-SY5Y Cells; Lane 5: SiHa Cells; Lane 6: 293 Cells; Lane 7: Hela Cells; Lane 8: PC-3M Cells; Lane 9: HepG2 Cells; Lane 10: A549 Cells; Probed with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Paraformaldehyde-fixed and paraffin-embedded Human Esophagus tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.


Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.