bsm-54491R-BF750

DATASHEET

Host: Rabbit

Target Protein: Phospho-Erk1(T202)+Erk2(T185)

Modification Site: Thr202+Thr185

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5595

Swiss Prot: P27361

Source: Synthetic phospho-Peptide corresponding to residues surrounding Thr185 of human Erk2.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].

Conjugation: AbBy Fluor-750

Excitation/ Emission: 749nm/775nm

Size: 100µL

Concentration: Lot Dependent

Applications: WB(WB(1:500-2000))
FCM(FCM(1:50-100))
IF(IHC-P)(IF(IHC-P)(1:50-200))
IF(ICC)(IF(ICC)(1:50-200))

Predicted Molecular Weight: 42/44

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.