VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human uterine neck; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with P-V-Myb+C-Myb(S11) Monoclonal Antibody, Unconjugated (bsm-54494R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with P-V-Myb+C-Myb(S11) Monoclonal Antibody, Unconjugated (bsm-54494R) at 1:200 for 30 minutes at room temperature, DAB staining.
Ags cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (P-V-Myb+C-Myb(S11)) Monoclonal Antibody, Unconjugated (bsm-54494R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Fig4: Flow cytometric analysis of MCF-7 cells with P-V-Myb+C-Myb(S11) antibody(bsm-54494R) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as antibody