bsm-54623R

DATASHEET

Host: Rabbit

Target Protein: Phospho-AKT1(S124)

Modification Site: Ser124

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 207

Swiss Prot: P31749

Source: Synthetic phospho-Peptide corresponding to residues surrounding Ser124 of human AKT1.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IP(1-2ug)
IHC(1:50-200)

Predicted Molecular Weight: 56

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.