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Host: Rabbit
Target Protein: TFRC
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 7037
Swiss Prot: P02786
Source: KLH conjugated synthetic peptide derived from human TFRC
Purification: Purified by Protein A.
Storage Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
Predicted Molecular Weight: 85
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFRC Monoclonal Antibody, Unconjugated (bsm-54633R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFRC Monoclonal Antibody, Unconjugated (bsm-54633R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFRC Monoclonal Antibody, Unconjugated (bsm-54633R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFRC Monoclonal Antibody, Unconjugated (bsm-54633R) at 1:400 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with TFRC monoclonal antibody, unconjugated (bsm-54633R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.