bsm-54653R [Primary Antibody]
GTPBP4 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GTPBP4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 23560

Swiss Prot: Q9BZE4

Source: Synthetic Peptide within N terminal human GTPBP4.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 74


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GTPBP4 Monoclonal Antibody, Unconjugated (bsm-54653R) at 1:50 for 30 minutes at room temperature, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GTPBP4 Monoclonal Antibody, Unconjugated (bsm-54653R) at 1:50 for 30 minutes at room temperature, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GTPBP4 Monoclonal Antibody, Unconjugated (bsm-54653R) at 1:50 for 30 minutes at room temperature, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GTPBP4 Monoclonal Antibody, Unconjugated (bsm-54653R) at 1:50 for 30 minutes at room temperature, DAB staining.


Flow cytometric analysis of GTPBP4 was done on thp-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54653R,1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).