Host: Rabbit
Target Protein: Tryptophanyl tRNA synthetase / WRS
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P23381
Source: Synthetic Peptide within C-terminal human Tryptophanyl tRNA synthetase/WRS.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 49/53
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody, Unconjugated (bsm-54661R) at 1:200 for 30 minutes at room temperature, DAB staining.
Flow cytometric analysis of Tryptophanyl tRNA synthetase / WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54661R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).