VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synapsin II Monoclonal Antibody, Unconjugated (bsm-54672R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bone marrow; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synapsin II Monoclonal Antibody, Unconjugated (bsm-54672R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synapsin II Monoclonal Antibody, Unconjugated (bsm-54672R) at 1:200 for 30 minutes at room temperature, DAB staining.
Flow cytometric analysis of Synapsin II was done on F9 cells. The cells were fixed, permeabilized and stained with Synapsin II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black)