bsm-54686R [Primary Antibody]
HER3/ErbB3 Phospho (pY1222) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HER3/ErbB3 Phospho (pY1222)

Modification Site: Tyr1222

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2065

Swiss Prot: P21860

Source: Synthetic phosphoPeptide corresponding to a region surrounding Tyrosine 1222 of Human ErbB3.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: Predicted band size: 148 .


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human thyroid; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HER3/ErbB3 Phospho (pY1222) Monoclonal Antibody, Unconjugated (bsm-54686R) at 1:100 for 30 minutes at room temperature, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HER3/ErbB3 Phospho (pY1222) Monoclonal Antibody, Unconjugated (bsm-54686R) at 1:100 for 30 minutes at room temperature, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HER3/ErbB3 Phospho (pY1222) Monoclonal Antibody, Unconjugated (bsm-54686R) at 1:100 for 30 minutes at room temperature, DAB staining.