bsm-54705R [Primary Antibody]
DBF4 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DBF4

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10926

Swiss Prot: Q9UBU7

Source: Synthetic Peptide within N-terminal human DBF4.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. Tissue specificity: Highly expressed in testis and thymus. Expressed also in most cancer cells lines.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 77


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54705R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).