bsm-54754R [Primary Antibody]
PDLIM1 Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PDLIM1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 9124

Swiss Prot: O00151

Source: Synthetic Peptide within N-terminal Human PDLIM1.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

PDLIM1 is a cytoskeletal protein that may act as an adapter that brings other proteins to the cytoskeleton. PDLIM1 is strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung, and minimally expressed in liver, thymus, kidney, prostate and pancreas. It is not found in the brain, testis, ovary, and peripheral blood leukocytes.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PDLIM1 Monoclonal Antibody, Unconjugated (bsm-54754R) at 1:50 for 30 minutes at room temperature, DAB staining.


HepG2 cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDLIM1 Monoclonal Antibody(bsm-54754R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).