DATASHEET
Host:
Rabbit
Target Protein:
PDLIM1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
9124
Swiss Prot:
O00151
Source:
Synthetic Peptide within N-terminal Human PDLIM1.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
PDLIM1 is a cytoskeletal protein that may act as an adapter that brings other proteins to the cytoskeleton. PDLIM1 is strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung, and minimally expressed in liver, thymus, kidney, prostate and pancreas. It is not found in the brain, testis, ovary, and peripheral blood leukocytes.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PDLIM1 Monoclonal Antibody, Unconjugated (bsm-54754R) at 1:50 for 30 minutes at room temperature, DAB staining.
HepG2 cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDLIM1 Monoclonal Antibody(bsm-54754R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).