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Host: Rabbit
Target Protein: CD44
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 960
Swiss Prot: P16070
Source: A synthesized peptide derived from human CD44
Purification: purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Conjugation: AbBy Fluor-647
Excitation/ Emission: 650nm/665nm
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:2000-20000)
IHC-F(1:200-2000)
IHC-P(1:200-2000)
ICC/IF(1:100-500)
IF(1:200-2000)
Predicted Molecular Weight: 85 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: CD44 Antibody, conjugated (bsm-54767R-BF647) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: CD44 Antibody, conjugated (bsm-54767R-BF647) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: CD44 Antibody, conjugated (bsm-54767R-BF647) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: CD44 Antibody, conjugated (bsm-54767R-BF647) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: CD44 Antibody, conjugated (bsm-54767R-BF647) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: CD44 Antibody, conjugated (bsm-54767R-BF647) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: CD44 Antibody, conjugated (bsm-54767R-BF647) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: CD44 Antibody, conjugated (bsm-54767R-BF647) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.