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Host: Rabbit
Target Protein: TGN46
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 10618
Swiss Prot: O43493
Source: KLH conjugated synthetic peptide derived from human TGN46
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: Lot Dependent
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IP(1-2ug)
IF
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human MDA-MB-231 cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human U251 cell lysates; Lane 6: Human A549 cell lysates probed with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TGN46 Monoclonal Antibody, Unconjugated (bsm-54769R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.