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Host: Rabbit
Target Protein: Caspase-3
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 836
Swiss Prot: P42574
Source: Recombinant human Caspase-3 protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications: WB(WB=1:1000-2000)
Predicted Molecular Weight: 32
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Jurkat (H) cells were treated with or without staurosporine (1uM) for 4 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Caspase-3 monoclonal antibody, unconjugated (bsm-55032R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.