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Host: Rabbit
Target Protein: MUC1/CD227
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4582
Swiss Prot: P15941
Source: Synthesized peptide derived from Human MUC1
Purification: Purified by Protein A.
Storage Buffer: PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with MUC1 monoclonal antibody, unconjugated (bsm-55523R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(bsm-55523R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.