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Host: Mouse
Target Protein: Transglutaminase 2
Clonality: Monoclonal
Isotype: IgG1
Entrez Gene: 7052
Source: Recombinant Human TGM2 protein
Purification: purified by Protein A.
Storage Buffer: PBS with 100 μg/ml BSA,0.15% ProClin300 and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µl
Concentration: Lot Dependent
Applications:
WB
IHC-F
IF(IHC-P)
IF(ICC)
IHC-P
Predicted Molecular Weight: 78
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with Transglutaminase 2 monoclonal antibody, unconjugated (bsm-55524M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (bsm-55524M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (bsm-55524M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (bsm-55524M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (bsm-55524M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Transglutaminase 2 Monoclonal Antibody, Unconjugated (bsm-55524M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed HUVEC (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Transglutaminase 2) monoclonal Antibody, unconjugated (bsm-55526M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.