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Host: Rabbit
Target Protein: CD4
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 920
Swiss Prot: P06332
Source: KLH conjugated synthetic peptide derived from mouse CD4
Purification: Purified by Protein A.
Storage Buffer: PBS with 0.02% Proclin300,100 μg/ml BSA and 50% glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:1000-5000))
IHC-P(IHC-P(1:200-500))
IHC-F(IHC-F(1:200-500))
IF(IHC-P)(IF(IHC-P)(1:200-500))
Predicted Molecular Weight: 51
Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with CD4 monoclonal antibody, unconjugated (bsm-55558R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-55558R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-55558R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-55558R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.