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Host: Rabbit
Target Protein: CD69
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 969
Swiss Prot: Q07108
Source: KLH conjugated synthetic peptide derived from human CD69
Purification: purified by Protein A.
Storage Buffer: 0.22μm filtered solution in 20mM hepes7.4, 150mm NaCl, 50% glycerin,0.03% proclin.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:500-2000)
IHC-P(1:100-200)
IHC-F(1:100-200)
IF(1:100-200)
IP(1:50-200)
ELISA(1:5000-20000)
Predicted Molecular Weight: 22 kDa
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Daudi (H) treated with PMA (50 ng/ml) for 24 h, 25 μg total protein per lane of cell lysates (see on figure) probed with CD69 monoclonal antibody, unconjugated (bsm-55564R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD69 Monoclonal Antibody, Unconjugated (bsm-55564R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.