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Host: Rabbit
Target Protein: DLK1
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 8788
Swiss Prot: P80370
Source: KLH conjugated synthetic peptide derived from human DLK1
Purification: purified by Protein A.
Storage Buffer: 0.22μm filtered solution in 20mM hepes7.4, 150mm NaCl, 50% glycerin,0.03% proclin.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
IHC-F(1:100-500)
IF(1:100-500)
IHC-P(1:100-500)
WB(1:500-2000)
Predicted Molecular Weight: 42 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DLK1 Monoclonal Antibody, Unconjugated(bsm-55569R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DLK1 Monoclonal Antibody, Unconjugated (bsm-55569R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with DLK1 monoclonal antibody, unconjugated (bsm-55569R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.