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Host: Rabbit
Target Protein: FSTL3
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 10272
Swiss Prot: O95633
Source: KLH conjugated synthetic peptide derived from human FSTL3
Purification: Purified by Protein A.
Storage Buffer: 0.22μm filtered solution in 20mM hepes7.4, 150mm NaCl, 50% glycerin,0.03% proclin.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
IHC-P(IHC-P(1:50-200))
IHC-F(IHC-F(1:50-200))
IF(IHC-P)(IF(IHC-P)(1:50-200))
IF(ICC)(IF(ICC)(1:50-200))
Predicted Molecular Weight: 28
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FSTL3) monoclonal Antibody, unconjugated (bsm-55571R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FSTL3 Monoclonal Antibody, Unconjugated(bsm-55571R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FSTL3 Monoclonal Antibody, Unconjugated(bsm-55571R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FSTL3 Monoclonal Antibody, Unconjugated(bsm-55571R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FSTL3 Monoclonal Antibody, Unconjugated(bsm-55571R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FSTL3 Monoclonal Antibody, Unconjugated(bsm-55571R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.