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Host: Rabbit
Target Protein: TNF-alpha
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 7124
Swiss Prot: P01375
Source: Recombinant human TNF alpha protein
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:1000-2000)
IHC-F(1:100-500)
IP(1:50-200)
IF(1:100-500)
IHC-P(1:100-500)
Predicted Molecular Weight: 26 kDa
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
THP-1 (H) cells were treated with or without LPS and Brefeldin A (5 µg/ml) for 4 h, 25 μg total protein per lane of cell lysates (see on figure) probed with TNF alpha monoclonal antibody, unconjugated (bsm-55603R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.