25 ug total protein per lane of various lysates (see on figure) probed with GJA1 | Connexin-43 monoclonal antibody, unconjugated (bsm-55612R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GJA1 | Connexin-43 Monoclonal Antibody, Unconjugated (bsm-55612R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.