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Host: Rabbit
Target Protein: GSDMD
Clonality: Recombinant
Entrez Gene: 79792
Swiss Prot: P57764
Source: Recombinant human GSDMD protein
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications:
WB
IHC-F
IF
IHC-P
Predicted Molecular Weight: 32/53
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
THP-1 (H) differentiated with TPA (50 ng/ml) for overnight and then LPS (5μg/ml) for 6 h, 25 μg total protein per lane of cell lysates (see on figure) probed with GSDMD monoclonal antibody, unconjugated (bsm-55614R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GSDMD Monoclonal Antibody, Unconjugated (bsm-55614R) at 1:600 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.