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Host: Rabbit
Target Protein: TUBA4A | alpha-Tubulin
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 7277
Swiss Prot: P68366
Source: KLH conjugated synthetic peptide derived from human TUBA4A
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:1000-2000)
ELISA(1:5000-10000)
IHC-F(1:200-800)
IP(1:50-100)
IF(1:200-800)
IHC-P(1:200-800)
ICC/IF(1:100-200)
Flow-Cyt(1:100-200)
Predicted Molecular Weight: 50 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with TUBA4A | alpha-Tubulin monoclonal antibody, unconjugated (bsm-55636R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human right temporal lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TUBA4A | alpha-Tubulin Monoclonal Antibody, Unconjugated (bsm-55636R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.