bsm-55673R [Primary Antibody]
CYP11A1 Recombinant Antibody
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Host: Rabbit

Target Protein: CYP11A1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 1583

Swiss Prot: P05108

Source: KLH conjugated synthetic peptide derived from human CYP11A1

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:1000-2000)
IHC-P(1:100-500)
IF(1:100-500)
IP(1:50-100)
ELISA(1:5000-10000)
IHC-F(1:100-500)

Predicted Molecular Weight: 53 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with CYP11A1 monoclonal antibody, unconjugated (bsm-55673R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CYP11A1 Monoclonal Antibody, Unconjugated (bsm-55673R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.