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Host: Rabbit
Target Protein: GAD65
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 2572
Swiss Prot: P48320
Source: Recombinant mouse GAD65 protein
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Applications:
WB(1:300-5000)
IHC-F(1:50-200)
IF(1:50-200)
IHC-P(1:50-200)
Predicted Molecular Weight: 65
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
25 ug total protein per lane of various lysates (see on figure) probed with GAD65 monoclonal antibody, unconjugated (bsm-55701R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-55701R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-55701R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GAD65 Monoclonal Antibody, Unconjugated (bsm-55701R) at 1:800 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.