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Host: Rabbit
Target Protein: phospho-AKT (Ser473)
Modification Site: (Ser473
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 207
Swiss Prot: P31749
Source: KLH conjugated Synthesised phosphopeptide derived from human AKT around the phosphorylation site of Ser473
Purification: Purified by Protein A.
Storage Buffer: PBS, Glycerol, BSA.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: Lot Dependent
Applications: WB(1:300-5000)
Predicted Molecular Weight: 56
Cross Reactive Species: Human
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
Jurkat (H) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-AKT (Ser473) monoclonal antibody, unconjugated (bsm-56203R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.