bsm-58004R
[Primary Antibody]
DYKDDDDK (10A2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: DYKDDDDK
Specificity: same epitope to Sigma FLAG tag
Clonality: Monoclonal
Isotype: IgG
Source: DYKDDDDK peptide
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
DYKDDDDK polypeptide tag (i.e. FLAG tag, currently owned by Sigma) is a common protein tag added to recombinant proteins. DYKDDDDK was originally used by Munro and Pelham in 1984. This artificially designed tag facilitates the identification and purification of recombinant proteins. The hydrophilic nature of DYKDDDDK make it less likely to denature or inactivate proteins to which it is fused. Moreover, N-terminal FLAG tags can be readily cleaved from proteins with enterokinase. It is one of the most widely used protein tags.
Size: 100ul
Concentration: Not Determined
Cross Reactive Species:
Human
All
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
293T transfected with DYKDDDDK protein, Lane 1: DYKDDDDK -protein 1 at 25ug; Lane 2: DYKDDDDK-protein 2 at 2ug; lysate was probed with DYKDDDDK (10A2) Monoclonal Antibody, Unconjugated (bsm-58004R) at 1 µg/ml dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
IF(ICC) staining with DYKDDDDK (10A2) Monoclonal Antibody (bsm-58004R) at 1:200 in 293T cells transfected with FLAG-GAPDH (green). The nuclear counterstain is DAPI (blue).
Flow cytometric analysis of 293T cells transfected with FLAG-PQBP1 with DYKDDDDK (10A2) Monoclonal Antibody (bsm-58004R) at a 1:100 dilution followed by secondary antibody incubation (orange), compared to primary antibody only without secondary antibody (blue), and secondary antibody only staining (red).